Efficient ultrafiltration-based protocol to deplete extracellular vesicles from fetal bovine serum
Fetal bovine serum (FBS) is the most commonly used supplement in studies involving cell-culture experiments.However, FBS contains large numbers of bovine extracellular vesicles (EVs), which hamper the analyses of secreted EVs from the cell type of preference and, thus, also the downstream analyses.Therefore, a prior elimination of EVs from FBS is c